Domenech-Mateu Collection Slide Scanning

Introduction

Slide Scanner 2019

Slide scanner delivered August, Zeiss technician installed at end of August, and test scanning commenced September 2019.

This page is designed to provide specific technical information for the slide scanner setup for collection scanning.

Embryo image file example: UAB-E25-03-2

UAB - identifies collection location
E25 - identifies New Embryo Reference
03 - identifies Slide number (note 3 digit coding could be used if more than 100 slides)
2 - used only if the slide needs scanning to capture sections in label region (close to slide holder).

Fetus image file example: UAB-F25-003-2

UAB - identifies collection location
F25 - identifies New Fetus Reference
003 - identifies Slide number (note 3 digit coding as these contain more than 100 slides)
2 - used only if the slide needs scanning to capture sections in label region (close to slide holder).

Sections should be scanned in the correct serial sequence
- This will be "upside down" compared to photocopied slide images.

Embryo E109 used as test series.

Embryo E109 30 mm (Carnegie Stage 23)

Week:	1	2	3	4	5	6	7	8
Carnegie stage:	1 2 3 4	5 6	7 8 9	10 11 12 13	14 15	16 17	18 19	20 21 22 23

Stage 23 Human Embryo (E109) 30 mm Abdomen (slide 1-17), thorax (slide 17-23), Original name - Bi-1

Slide 1-42

Additional slides

Also a "B" series? slide 8 - 23 and 55?

Slides cleaned with microfibre cloth, excess mountain removed with blade.
Sticker added to label zone with embryo number and slide number.
Naming UAB-E109-slidenumber
Need to reset sequence of scan/numbering - bottomtopleftright (will this be different for different embryos?)
Course autofocus not working correctly - need to manually set (3300-3400)
Slide 1 image 15,16 not sharp; slide 2 image 13 not sharp. When rescanned after fine focus on these section and wider course range OK.