UAB-DMC 2019 Slide Scanning: Difference between revisions

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=Domenech-Mateu Collection Slide Scanning=
=Domenech-Mateu Collection Slide Scanning=
[[Domenech-Mateu Collection]]
==Introduction==
==Introduction==
[[File:Domenech-Mateu Collection-Scanner2019-01.jpg|thumb|alt=Domenech-Mateu Collection-Scanner2019|Slide Scanner 2019]]
[[File:Domenech-Mateu Collection-Scanner2019-01.jpg|thumb|alt=Domenech-Mateu Collection-Scanner2019|Slide Scanner 2019]]
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Embryo E109 30 mm (Carnegie Stage {{CS23}})
==Image Coding==


[[File:Domenech-Mateu Collection-Scanner2019-02.jpg|600px]]
Embryo image file example: UAB-E25-03-2
 
* '''UAB''' - identifies collection location
* '''E25''' - identifies New Embryo Reference
* '''03''' - identifies Slide number (note 3 digit coding could be used if more than 100 slides)
* '''2''' - used only if the slide needs scanning to capture sections in label region (close to slide holder).
 
Fetus image file example: UAB-F25-003-2
 
* '''UAB''' - identifies collection location
* '''F25''' - identifies New Fetus Reference
* '''003''' - identifies Slide number (note 3 digit coding as these contain more than 100 slides)
* '''2''' - used only if the slide needs scanning to capture sections in label region (close to slide holder).
 
* Sections should be scanned in the correct serial sequence
** This will be "upside down" compared to photocopied slide images.
 
 
==Preparation Notes==
* Slides cleaned with microfibre cloth, excess mountain removed with blade.
* Sticker added to label zone with embryo number and slide number.
* Naming UAB-E109-slidenumber
* Need to reset sequence of scan/numbering - bottomtopleftright (will this be different for different embryos?)
* Course autofocus need to manually set (3300-3400) then save.
* Embryo E109 used as first test series.




[[File:Domenech-Mateu Collection-Scanner2019-02.jpg|600px]]


{{Carnegie_stage_table_1}}
{{Carnegie_stage_table_1}}

Latest revision as of 00:55, 18 September 2019

Domenech-Mateu Collection Slide Scanning

Domenech-Mateu Collection

Introduction

Domenech-Mateu Collection-Scanner2019
Slide Scanner 2019

Slide scanner delivered August, Zeiss technician installed at end of August, and test scanning commenced September 2019.

This page is designed to provide specific technical information for the slide scanner setup for collection scanning.


Image Coding

Embryo image file example: UAB-E25-03-2

  • UAB - identifies collection location
  • E25 - identifies New Embryo Reference
  • 03 - identifies Slide number (note 3 digit coding could be used if more than 100 slides)
  • 2 - used only if the slide needs scanning to capture sections in label region (close to slide holder).

Fetus image file example: UAB-F25-003-2

  • UAB - identifies collection location
  • F25 - identifies New Fetus Reference
  • 003 - identifies Slide number (note 3 digit coding as these contain more than 100 slides)
  • 2 - used only if the slide needs scanning to capture sections in label region (close to slide holder).
  • Sections should be scanned in the correct serial sequence
    • This will be "upside down" compared to photocopied slide images.


Preparation Notes

  • Slides cleaned with microfibre cloth, excess mountain removed with blade.
  • Sticker added to label zone with embryo number and slide number.
  • Naming UAB-E109-slidenumber
  • Need to reset sequence of scan/numbering - bottomtopleftright (will this be different for different embryos?)
  • Course autofocus need to manually set (3300-3400) then save.
  • Embryo E109 used as first test series.


Domenech-Mateu Collection-Scanner2019-02.jpg

Week: 1 2 3 4 5 6 7 8
Carnegie stage: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23